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- Smarter Race Cdna Amplification Kit User Manual Download
- Smarter Race Cdna Amplification Kit User Manual Pdf
Smarter Race Cdna Amplification Kit User Manual Download
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Smarter Race Cdna Amplification Kit User Manual Pdf
Could you explain about how to make long distance PCR (LD PCR) from RACE PCR by using 5'cDNA as template? I have read the protocol of SMARTer RAE5'/3' Kit User Manual, but I can not understand. SMARTer™ RACE cDNA Amplification Kit User Manual III. Introduction & Protocol Overview The SMARTer™ RACE cDNA Amplification Kit provides a method for performing both 5’- and 3’-rapid amplification of cDNA ends (RACE). The SMARTer RACE cDNA Amplification Kit is an improved version. Aug 22, 2016 Advantages of SMARTer Technology in RACE Applications With the SMARTer RACE cDNA Amplification Kit, the SMARTer II A Oligonucleotide is added. A Takara Bio Company Page 28 of 30 SMARTer? RACE 5’/3’ Kit User Manual Appendix D. 5’-RACE cDNA Amplification with Random Primers If your RNA template is from a non-eukaryotic organism and/or lacks a polyadenylated tail, use the following protocol for first-strand cDNA synthesis with random priming. Sep 20, 2015 5’- rapid amplification of cDNA ends (5’ RACE) assay 5’ ends of the oncogenes cDNA were amplified by SMARTer™ RACE cDNA Amplification Kit. First-Strand cDNA was generated by SMARTScribe™ Reverse Transcriptase and primed using SMARTer II A Oligonucleotide and 5'-CDS Primer A. The following reagents were added in one tube 2.75 µl mRNA.